세포부착을 위한 스캐폴드 개발 및 줄기세포를 적용한 스캐폴드의 조직재생능력 평가

유훈; 송경호; 임현창; 이중석; 윤정호; 서영권; 정의원; 이용근; 오남식; 최성호

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세포부착을 위한 스캐폴드 개발 및 줄기세포를 적용한 스캐폴드의 조직재생능력 평가
Development of Scaffold for Cell Attachment and Evaluation of Tissue Regeneration Using Stem Cells Seeded Scaffold

대한구강악안면임플란트학회지 2014년 18권 2호 p.120 ~ 138

유훈, 송경호, 임현창, 이중석, 윤정호, 서영권, 정의원, 이용근, 오남식, 최성호,

소속 상세정보

유훈 ( You Hoon ) - 연세대학교 치과대학 치주과학교실
송경호 ( Song Kyung-Ho ) - 연세대학교 치과대학 치주과학교실
임현창 ( Lim Hyun-Chang ) - 연세대학교 치과대학 치주과학교실
이중석 ( Lee Jung-Seok ) - 연세대학교 치과대학 치주과학교실
윤정호 ( Yun Jeong-Ho ) - 연세대학교 치과대학 치주과학교실
서영권 ( Seo Young-Kwon ) - 동국대학교 생명화학공학과
정의원 ( Jung Ui-Won ) - 연세대학교 치과대학 치주과학교실
이용근 ( Lee Yong-Keun ) - (주)예스바이오골드
오남식 ( Oh Nam-Sik ) - 인하대학교 의과대학 치과학교실
최성호 ( Choi Seong-Ho ) - 연세대학교 치과대학 치주과학교실

KMID : 0602720140180020120

Abstract

Purpose: The purpose of this study was to review the outcomes of a series of studies on tissue regeneration conducted in multiple institutions including the Department of Periodontology, College of Dentistry, Yonsei University.

Materials and Methods: Studies were performed divided into the following three subjects; 1) Development of three-dimensional nano-hydroxyapatite (n-HA) scaffold for facilitating drug release and cell adhesion. 2) Synergistic effects of bone marrow-derived mesenchymal stem cells (BMMSC) application simultaneously with platelet-rich plasma (PRP) on HA scaffolds. 3) The efficacy of silk scaffolds coated with n-HA. Also, all results were analyzed by subjects.

Results: Hollow hydroxyapatite spherical granules were found to be a useful tool for the drug release and avidin-biotin binding system for cell attachment. Also, BMMSC simultaneously with PRP applied in an animal bone defect model was seen to be more synergistic than in the control group. But, the efficacy of periodontal ligament cells and dental pulp cells with silk scaffolds could not be confirmed in the initial phase of bone healing.

Conclusion: The ideal combination of three elements of tissue engineering?scaffolds, cells and signaling molecules could be substantiated due to further investigations with the potentials and limitations of the suggested list of studies.